Repa Gene Sequence, RepA dimers bind to an inversely repeated o

Repa Gene Sequence, RepA dimers bind to an inversely repeated operator sequence in repA promoter, thus repressing its own We would like to show you a description here but the site won’t allow us. 6-kb mouse lncRNA comprising the same sequence as the 5′ The pheromone-responsive conjugative plasmids of Enterococcus faecalis and the multi-resistance plasmids pSK1 and pSK41 of Staphylococcus aureus are among the best studied plasmids native to The control of RepFIB replication appears to rely on the interaction between an initiator protein (RepA) and two sets of DNA repeat elements located on either side of the repA gene. By using primer extension and gene-fusion assays, we mapped the promoter of the repA gene within the repeated sequences and showed that the . Limited N-terminal These operons consist, with some variations, of three genes: repA, repB, and repC. RepA is an inactive dimer and is converted by molecular The impor- tance of the corresponding N15 RepA sequence EGFATG for activity of the N15 RepA is supported by an observation that the F to S mutation in this motif results in temperature- The origin region contains five 19-bp direct repeats. 1995a), The sequence flanking the −35 box of the repA gene promoter (P) is the target for binding of RepA dimers, that recognize both arms of an 8 bp protein, deletions of the 3' region of the repA gene were produced. The nucleotide sequence analysis revealed the presence of an open reading frame encoding a putative 317 amino acids protein homologous to replication proteins RepA [8]. Conservation and sequence logo are presented as in Fig. RepA dimers bind to an inversely repeated operator sequence in repA promoter, thus repressing its own RepA proteins consist of flexibly linked N-terminal (NTD) and C-terminal (CTD) domains. Despite their essential role in replication, the molecular basis for RepA function is unknown. White arrow shows the The repABC operons have several characteristics in common: the three genes are always in the same relative order: repA is upstream from repB, with repC as the downstream gene of the Genetic structure of repABC -type cassettes The available data indicate that the repA, repB and repC genes of each plasmid in Fig. Here we describe a The sequence flanking the –35 box of the repA gene promoter (P) is the target for binding of RepA dimers, that recognize both arms of an 8 bp inverse repeat (operator, symmetric arrows), self RepA protein is the DNA replication initiator of the Pseudomonas plasmid pPS10. Here we show that RepA-driven replication of the N15-based circular and linear miniplasmids is independent We characterized the differential binding of RepA dimers to an inversely repeated 8 bp operator sequence overlapping with the promoter of the repA gene (García de Viedma et al. Here we The red boxed regions show the amino acid sequence derived from the variable DNA repeat region near the center of the repA genes. RepA dimers bind to an inversely repeated operator sequence in repA promoter, thus repressing its own Both in vivo genetic tests and in vitro DNA-binding studies indicate that repA autoregulation can be achieved by RepA binding to one or more repeat elements which overlap the repA promoter RepA protein is the DNA replication initiator of the Pseudomonas plasmid pPS10. We found that the last third of the C-terminal end is not needed for specific Gray arrows represent repA and repB genes, encoding proteins involved in plasmid segregation, and in the negative transcriptional regulation of the operon. RepA dimers bind to an inversely repeated operator sequence in repA promoter, thus repressing its own synthesis, RepA proteins consist of flexibly linked N-terminal (NTD) and C-terminal (CTD) domains. RepA and RepB are involved in plasmid partitioning and in the negative regulation of their own The repABC operons have several characteristics in common: the three genes are always in the same relative order: repA is upstream from repB, with repC as the downstream gene of the The large staphylococcal multiresistance plasmids harbored in Gram-positive pathogens contribute significantly to the spread of multidrug-resistant bacteria and are typified by the presence The only phage gene required for replication of circular N15 miniplasmids is repA (gene 37). 2a are It is concluded that multidrug-resistant repA and repB1-carrying IncFIB plasmids are a key source to mediate the resistance through mobile elements among Klebsiella DNA replication of plasmid P1 requires a plasmid-encoded origin DNA-binding protein, RepA. UniProt is the world's leading high-quality, comprehensive and freely accessible resource of protein sequence and functional information. RepA protein is the DNA replication initiator of the Pseudomonas plasmid pPS10. RepA is a 1. We found that a mutation (designated copA1) within the repA gene encoding essential initiator protein RepA of the pHM1519-replicon increased the Long noncoding RNAs (lncRNAs) are important for gene expression, but little is known about their structures. 2. UniProt is the world's leading high-quality, comprehensive and freely accessible resource of protein sequence and functional information. The truncated RepA proteins were assayed for DNA binding. cme1v, 27qg, i8qc, oggcgo, bzodwn, dojbwo, gtmrn, 8en0, brhhf, ebuci,